Work

A bacterial pore-forming toxin forms aggregates in cells that resemble those associated with neurodegenerative diseases

Year: 2008

Type: article

Abstract: Listeria monocytogenes is a bacterial, facultative intracellular pathogen, which secretes a pore-forming toxin called listeriolysin O (LLO). LLO mediates the dissolution of the phagosomal membrane allowing L. monocytogenes to reach and grow in the host cytosolic compartment. In this study we report the localization of LLO secreted in infected cells. We described that LLO (i) forms small perinuclear aggregates, (ii) accumulates in large autophagosome-like structures and (iii) sequesters to large protein aggregates. The formation of protein aggregates required full LLO activity. Further characterization of protein aggregates indicated that they not only contained the active form of LLO but also polyubiquitinated proteins and p62, which are both common components of protein aggregates found in neurological diseases. Hence, a protein of bacterial origin could potentially follow the same fate as a toxic protein associated with neurodegenerative disease.

Source: Cellular Microbiology

DOI: 10.1111/j.1462-5822.2007.01100.x

Authors: Julie P. M. Viala, Sofia N. Mochegova, Nicole Meyer-Morse, Daniel A. Portnoy

Institutions: Centre National de la Recherche Scientifique, Laboratoire de Chimie Bactérienne, University of California, Berkeley

Language: English

Topic: Autophagy in Disease and Therapy

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Cerus Corporation, National Institutes of Health, National Institute of Allergy and Infectious Diseases