Year: 2000
Type: article
Abstract: A coliphage named 63D, isolated previously, associated sialidase as a component of phage particles. In order to localize the enzyme in phage particles, phages were partially destroyed by sonication, and the disrupted particles were size fractionated using a sucrose density gradient. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay and electron micrography of the fractions revealed the enzyme to be composed of four identical subunits with a molecular mass of 90 kDa, and the subunits were cross-linked by disulfide bonds. Electron micrographic observation indicated that six enzyme molecules were localized in a phage tail plate as a hexagonal array.
Source: FEMS Microbiology Letters
Authors: Yuichi Machida, Katsuhide Miyake, Kouji Hattori, Shin Yamamoto, Mitsuo Kawase, Shinji Iijima
Institutions: Nagoya University, NGK Insulators (Japan)
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W1964761329