Work

Detection of Australia Antigen by Radioimmunoassay

Year: 1971

Type: article

Abstract: A double-antibody radioimmunoassay has been developed for the detection of Australia antigen and antibodies to Australia antigen in human sera. The assay is simple to perform, and can be completed in 18 hr. Based on parallel studies of human sera, it is approximately 2000 times more sensitive than agar gel diffusion and 200 times more sensitive than quantitative complement fixation. Australia antigen or antibody to Australia antigen was demonstrated in 22 of 23 single serum samples obtained from patients acutely ill with the long-incubation form of hepatitis; eight of the sera were negative when evaluated by less sensitive techniques.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.68.5.1056

Authors: Richard D. Aach, Joe W. Grisham, Charles W. Parker

Institution: Washington University in St. Louis

Language: English

Topic: Hepatitis B Virus Studies

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: John A. Hartford Foundation, Hartford Foundation for Public Giving, U.S. Public Health Service