Work

Excision of prophage lambda in a cell-free system.

Year: 1975

Type: article

Abstract: A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of the substrate DNA undergoes recombination in 30 min. There is an absolute requirement for ATP; Mg++ and spermidine are stimulatory. RNA does not appear to be involved, nor can a role for DNA synthesis be demonstrated.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.72.6.2188

Authors: S Gottesman, M Gottesman

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W1964956319

FWCI: 11.8

Cites: 10

Cited by: 40

Related to: 10