Binary Probes for Nucleic Acid Analysis
Year: 2010
Type: review
Abstract: The advances in the development of binary probe (BPs) and their improved selectivity in comparison with other hybridization-based techniques were studied. The first BP, which used Förster resonance energy transfer (FRET), was suggested in 1988. A commonly adopted BP architecture employs the different affinity mode BPs. In this design one strand with a longer analyte binding arm binds tightly to the position abutting to the single-nucleotide polymorphisms (SNP) site. A second shorter analyte binding arm interrogates the SNP site by forming stable hybrid only with the perfectly matched sequence. The design of BPs employs self-assembly of more than two nucleic acid components. The same principle is adopted by DNA nanotechnology, which deals with constructing objects and functionally active assemblies from DNA molecules. Newly designed constructs based on aptamers, DNA junctions, and DNA enzymes offer an opportunity to utilize DNA probes that avoid direct covalent attachment with organic dyes.
Source: Chemical Reviews
DOI: 10.1021/cr900323b
Author: Dmitry M. Kolpashchikov
Institution: University of Central Florida
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1965800165