Work

Protein secondary structure determination by NMR Application with recombinant human cyclophilin

Year: 1991

Type: article

Abstract: It is a unique trait of the NMR method for protein structure determination that a description of the polypeptide secondary structure can be obtained at an early stage and quite independently of the complete structure calculation. In this paper the procedures used for secondary structure determination are reviewed and placed in perspective relative to the other steps in a complete three-dimensional structure determination. As an illustration the identification of the regular secondary structure elements in human cyclophilin is described.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)80808-g

Authors: Kurt Wüthrich, Claus Spitzfaden, Klaus Memmert, Hans Widmer, Gerhard Wider

Institutions: ETH Zurich, Institute of Molecular Biology and Biophysics, Sandoz Group AG (Switzerland)

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Award: Anwendung und Weiterentwicklung der NMR-Methode zur Protein- strukturermittlung