Work

Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids

Year: 2000

Type: article

Abstract: The application of high-performance liquid chromatography (HPLC) using a C30 reverse-phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana, and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.

Source: The Plant Journal

DOI: 10.1111/j.1365-313x.2000.00896.x

Authors: Paul D. Fraser, M. Elisabete S. Pinto, Daniel E. Holloway, Peter M. Bramley

Institutions: University of London, Royal Holloway University of London

Language: English

Topic: Plant biochemistry and biosynthesis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Royal Society, Biotechnology and Biological Sciences Research Council

OpenAlex ID: W1965916795

FWCI: 2.986

Cites: 34

Cited by: 413

Related to: 10