Exhaled breath condensate adenosine tracks lung function changes in cystic fibrosis
Year: 2013
Type: article
Study design: Observational Study
Abstract: Measurement of exhaled breath condensate (EBC) biomarkers offers a noninvasive means to assess airway disease, but the ability of EBC biomarkers to track longitudinal changes in disease severity remains unproven. EBC was collected from pediatric patients with cystic fibrosis (CF) during regular clinic visits over 1 yr. EBC biomarkers urea, adenosine (Ado), and phenylalanine (Phe) were measured by mass spectrometry, and biomarker ratios were used to control for variable dilution of airway secretions. EBC biomarker ratios were assessed relative to lung function in longitudinal, multivariate models and compared with sputum inflammatory markers and quality of life assessment (CFQ-R). EBC was successfully analyzed from 51 subjects during 184 visits (3.6 ± 0.9 visits per subject). EBC Ado/urea ratio was reproducible in duplicate samples (r = 0.62, P < 0.01, n = 20) and correlated with sputum neutrophil elastase (β = 2.5, P < 0.05). EBC Ado/urea correlated with the percentage predicted of forced expiratory volume in 1 s in longitudinal, multivariate models (β = -2.9, P < 0.01); EBC Ado/Phe performed similarly (β = -2.1, P < 0.05). In contrast, IL-8 and elastase measured in spontaneously expectorated sputum (n = 57 samples from 25 subjects) and the CFQ-R respiratory scale (n = 90 tests from 47 subjects) were not significantly correlated with lung function. EBC was readily collected in a clinic setting from a wide range of subjects. EBC Ado tracked longitudinal changes in lung function in CF, with results similar to or better than established measures.
Institution: University of North Carolina at Chapel Hill
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1632539253, G2224920561, G2258471767, Purines as Biomarkers of Respiratory Disease, Pulmonary Epithelia in Health and Disease, G3367022616, Pulmonary Surface Liquid Homeostasis, G6182122432, G6187953430, UNC Clinical and Translational Science Award, Mucus Adhesion to Epithelial Cells and its Mobilization in COPD and CF, UNC Center for Environmental Health and Susceptibility, UNC Cystic Fibrosis Research and Translation Core Center
OpenAlex ID: W1965933003