Work

Preparative Separation of High-purity Cordycepin From Cordyceps Militaris(l.) Link by High-speed Countercurrent Chromatography

Year: 2011

Type: article

Abstract: A high-speed counter-current chromatography (HSCCC) technique in a preparative scale has been applied to separate and purify cordycepin from the extract of Cordyceps militaris(L.) Link by a one-step separation. A high efficiency of HSCCC separation was achieved on a two-phase solvent system of n-hexane-n-butanol-methanol-water (23:80:30:155, v/v/v/v) by eluting the lower mobile phase at a flow rate of 2 ml/min under a revolution speed of 850 rpm. HSCCC separation of 216.2 mg crude sample (contained cordycepin at 44.7% purity after 732 cation-exchange resin clean-up) yielded 64.8 mg cordycepin with purity of 98.9% and 91.7% recovery. Identification of the target compound was performed by UV, IR, MS, (1)H NMR and (13)C NMR.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826076.2011.556965

Authors: Xie Huichun, Yong Liang, Deqiang Lao, Tianyou Zhang, Yoichiro Ito

Institutions: South China Normal University, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009