X-ray scattering in the plane of membrane
Year: 1993
Type: article
Abstract: We demonstrate a technique for measuring x-ray (or neutron) scattering with the momentum transfer oriented in the plane of a membrane, for the purpose of studying the lateral organization of proteins and peptides. As an example, the controversial question of whether gramicidin forms aggregates in membrane was investigated. We measured dilauroylphosphatidylcholine (DLPC) bilayers containing gramicidin in the molar ratio of 10:1. Very clear scattering curves reflecting gramicidin channel-channel correlation were obtained. Analysis of the data shows that the channels were randomly distributed in the membrane. We suggest that oriented proteins may provide substantial x-ray contrast against the lipid background without requiring heavy-atom labeling. This should open up many possible new experiments.
DOI: 10.1051/jp4:1993852
Authors: K. HE, S. J. LUDTKE, Y. WU, H. W. HUANG
Institution: Rice University
Language: French
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1966393487