Work

Analysis of Cooperativity by Isothermal Titration Calorimetry

Year: 2009

Type: article

Abstract: Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms10083457

Author: Alan Brown

Institution: University of Cambridge

Language: English

Topic: thermodynamics and calorimetric analyses

Subfield: Physical and Theoretical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,499

Funders: Wellcome Trust, Biotechnology and Biological Sciences Research Council