Work

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

Year: 2007

Type: article

Abstract: An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.2717684

Authors: Veronica Gomez-Godinez, Nicole Wakida, Alexander S. Dvornikov, Kyoko Yokomori, Michael W. Berns

Institution: University of California, Irvine

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze