Work

Ribonucleoprotein immunoprecipitation (RNP‐IP): A direct in vivo analysis of microRNA‐targets

Year: 2010

Type: article

Abstract: We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.

Source: Journal of Cellular Biochemistry

DOI: 10.1002/jcb.22562

Authors: Mohammad Q. Hassan, Jonathan A.R. Gordon, Jane B. Lian, Andre J. van Wijnen, Janet L. Stein, Gary S. Stein

Institution: University of Massachusetts Chan Medical School

Language: English

Topic: MicroRNA in disease regulation

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Dental and Craniofacial Research