Virus-based Photo-Responsive Nanowires Formed By Linking Site-Directed Mutagenesis and Chemical Reaction
Year: 2013
Type: article
Abstract: Owing to the genetic flexibility and error-free bulk production, bio-nanostructures such as filamentous phage showed great potential in materials synthesis, however, their photo-responsive behaviour is neither explored nor unveiled. Here we show M13 phage genetically engineered with tyrosine residues precisely fused to the major coat protein is converted into a photo-responsive organic nanowire by a site-specific chemical reaction with an aromatic amine to form an azo dye structure on the surface. The resulting azo-M13-phage nanowire exhibits reversible photo-responsive properties due to the photo-switchable cis-trans isomerisation of the azo unit formed on the phage. This result shows that site-specific display of a peptide on bio-nanostructures through site-directed genetic mutagenesis can be translated into site-directed chemical reaction for developing advanced materials. The photo-responsive properties of the azo-M13-phage nanowires may open the door for the development of light controllable smart devices for use in non-linear optics, holography data storage, molecular antenna, and actuators.
Source: Scientific Reports
DOI: 10.1038/srep01820
Institution: University of Oklahoma
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: National Science Foundation, U.S. Department of Defense, Oklahoma Center for the Advancement of Science and Technology, National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research, Division of Materials Research, Division of Chemical, Bioengineering, Environmental, and Transport Systems, Division of Civil, Mechanical and Manufacturing Innovation
Awards: G1148302489, Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and Therapy, G1509628204, ANTI-COCAINE BRAIN-HOMING VIRUS-LIKE NANOPARTICLES, Collaborative Research: Chemical and Biological Quantum Nanosensors Based on Nanoparticle Molecules, G2182270004, Bone-seeking and cell-targeting non-viral vectors for BMP-2 gene delivery, Complex Nanocomposites for Bone Regeneration, G4119534169, G5324093878, G5508127933, G5841752660, G5850682332, CAREER: Genetically Modifiable Shape-Tunable Protein Nanotubes as Templates for Controlled Nano-Synthesis and Assembly, G6204873114, G6490638229, Gene delivery vectors inspired from the structure and assembly process of target-recognizing phage, Biomineralization and self-assembly of genetically modifiable nanofibers to build bone tissue engineering scaffolds, G7332541666
OpenAlex ID: W1968522312