Year: 2009
Type: article
Abstract: Understanding how a small brain region, the suprachiasmatic nucleus (SCN), can synchronize the body's circadian rhythms is an ongoing research area. This important time-keeping system requires a complex suite of peptide hormones and transmitters that remain incompletely characterized. Here, capillary liquid chromatography and FTMS have been coupled with tailored software for the analysis of endogenous peptides present in the SCN of the rat brain. After ex vivo processing of brain slices, peptide extraction, identification, and characterization from tandem FTMS data with <5-ppm mass accuracy produced a hyperconfident list of 102 endogenous peptides, including 33 previously unidentified peptides, and 12 peptides that were post-translationally modified with amidation, phosphorylation, pyroglutamylation, or acetylation. This characterization of endogenous peptides from the SCN will aid in understanding the molecular mechanisms that mediate rhythmic behaviors in mammals.
Source: Molecular & Cellular Proteomics
Authors: Ji Eun Lee, Norman Atkins, Nathan G. Hatcher, Leonid Zamdborg, Martha U. Gillette, Jonathan V. Sweedler, Neil L. Kelleher
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
Funders: David and Lucile Packard Foundation, Alfred P. Sloan Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute on Drug Abuse, NIH Office of the Director, National Institute of General Medical Sciences, National Institute of Dental and Craniofacial Research
OpenAlex ID: W1968820863