Work

Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Year: 1997

Type: article

Abstract: Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns-such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery-were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.

Source: Journal of Chromatography A

DOI: 10.1016/s0021-9673(97)00254-9

Authors: Shaofeng Xie, Frantisek Svec, Jean M.J Fréchet

Institutions: Cornell University, University of California, Berkeley

Language: English

Topic: Analytical Chemistry and Chromatography

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,040

Funder: National Institute of General Medical Sciences