Work

Construction and Transposition of a 100-kilobase Extended P Element in Drosophila

Year: 2000

Type: preprint

Abstract: We have used P element deletion derivatives at defined locations in the Drosophila genome to construct a 100-kb extended P element more than twice the size of any previously available. We demonstrate that this prototypical extended P element is capable of transposition to new sites in the genome. The structural and functional integrity of a transposed extended P element was confirmed using molecular, genetic, and cytogenetic criteria. This is the first method shown to be capable of producing large, unlinked transpositional duplications in Drosophila. The ability to produce functional transposable elements from half-elements is novel and has many potential applications for the functional analysis of complex genomes.

Source: Genome Research

DOI: 10.1101/gr.151700

Authors: Brian C. Ring, Hank W. Bass, Dan Garza

Institution: Florida State University

Language: English

Topic: Chromosomal and Genetic Variations

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: Florida State University, National Institutes of Health, National Human Genome Research Institute

Awards: METHODS FOR THE MANIPULATION OF THE DROSOPHILA GENOME, G8358310327