Work

Characterization of MAD2B and Other Mitotic Spindle Checkpoint Genes

Year: 1999

Type: article

Abstract: Aneuploidy is a characteristic of the majority of human cancers, and recent work has suggested that mitotic checkpoint defects play a role in its development. To further explore this issue, we isolated a novel human gene, MAD2B (MAD2L2), which is homologous to the spindle checkpoint gene MAD2 (MAD2L1). We determined the chromosomal localization of it and other spindle checkpoint genes, including MAD1L1, MAD2, BUB3, TTK (MPS1L1), and CDC20. In addition, we resolved the genomic intron-exon structure of the human BUB1 gene. We then searched for mutations in these genes in a panel of 19 aneuploid colorectal tumors. No new mutations were identified, suggesting that genes yet to be discovered are responsible for most of the checkpoint defects observed in aneuploid cancers.

Source: Genomics

DOI: 10.1006/geno.1999.5831

Authors: Daniel P. Cahill, Luis T. da Costa, Eleanor B. Carson-Walter, Kenneth W. Kinzler, Bert Vogelstein, Christoph Lengauer

Institutions: Johns Hopkins University, Johns Hopkins Medicine, Howard Hughes Medical Institute

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,980