Work

Interference of nonsense mutations with eukaryotic messenger RNA stability.

Year: 1979

Type: article

Abstract: The fine structure map of the yeast URA 3 gene was established by meiotic recombination, and amber nonsense mutations were located at different points on the map. The effect of the length of the labeling time on the specific radioactivity of ura 3 messenger RNA and on its repartition between poly(A)-RNA and RNA not containing poly(A) has been followed in nonsense mutants. Nonsense mutations reduce the messenger level without lowering its instantaneous rate of synthesis. The strength of the reduction depends on the position of the nonsense codon within the locus and concerns essentially the accumulation of polyadenylylated ura 3 mRNA.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.76.10.5134

Authors: R Losson, F Lacroute

Institutions: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire et Cellulaire

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Centre National de la Recherche Scientifique

OpenAlex ID: W1971724293

FWCI: 2.633

Cites: 23

Cited by: 473

Related to: 10