Work

A Fluorescent Reporter of the Phosphorylation Status of the Substrate Protein STAT3

Year: 2011

Type: article

Abstract: Roving reporter: A reversible biosensor reports the phosphorylation status of full-length substrate protein STAT3 by genetically incorporating 7-hydroxycoumarin into its phosphotyrosine binding pocket (see picture). A large fluorescence increase with characteristic emission and excitation properties results as a response to phosphorylation of STAT3 in vitro and endogenously in nuclear extracts.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201102923

Authors: Vanessa K. Lacey, Angela R. Parrish, Shuliang Han, Zhouxin Shen, Steven P. Briggs, Yuguo Ma, Lei Wang

Institutions: Salk Institute for Biological Studies, Peking University, University of California San Diego

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: March of Dimes Foundation, National Institutes of Health, National Cancer Institute

Awards: G109926362, Genetically Encoding Novel Amino Acids to Investigate Wnt Signaling in C. elegans, Cancer Center Support Grant (CCSG)

OpenAlex ID: W1971823130

FWCI: 3.159

Cites: 30

Cited by: 37

Related to: 10