Work

Ectopic expression of soybean methionine synthase delays flowering time in transgenic tobacco plants

Year: 2014

Type: article

Abstract: A photoperiod-sensitive soybean [Glycine max (L.) Merr] cv. ZhongDou 24 (ZD24) exhibiting delayed flowering when grown under long-days (LD, a 16-h photoperiod) was used to identify the genetic control of flowering delay. A differential expression profiling technique enabled identification of a gene fragment that was up-regulated under LD. This fragment was homologous to a gene encoding methionine synthase (MS) in soybean and was named GmMS. The RNA content confirmed that GmMS was expressed in roots, stems, and leaves of soybean grown under LD. The highest expression was in stems. Full length GmMS, encoding 763 amino acids, was transferred into tobacco plants. The ectopic expression of GmMS in tobacco resulted in delayed flowering. Other effects included stunting, an increased MS activity and methionine content, a higher content of alcohol-soluble proteins and of chlorophylls, and a lower content of anthocyanins.

Source: Biologia Plantarum

DOI: 10.1007/s10535-014-0461-2

Authors: A. H. Sha, Z. L. Gao, H. Wu, D. Z. Lin, Q. L. Zhang, Y. H. Chen

Institutions: Yangtze University, Hainan University

Language: English

Topic: Plant Molecular Biology Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: National Natural Science Foundation of China, Specialized Research Fund for the Doctoral Program of Higher Education of China