Work

Ribosyl Geometry in the Transition State of Streptococcus pneumoniae Methylthioadenosine Nucleosidase from the 3′-2H Kinetic Isotope Effect

Year: 2008

Type: article

Abstract: Synthesis of [3'-2H]-labeled 5'-methylthioadenosine (MTA) derivatives permitted measurement of the [3'-2H] KIE of the reaction catalyzed by Streptococcus pneumoniae methylthioadenosine nucleosidase (spMTAN). The key [3'-2H] KIE revealed the partial 3'-OH polarization and H3'-endo-->exo ribosyl configuration at the spMTAN transition state. A new understanding of the transition state stabilization of spMTAN-catalyzed hydrolysis is uncovered in structural features at the spMTAN transition state.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja804578m

Authors: Minkui Luo, Vern L. Schramm

Institution: Albert Einstein College of Medicine

Language: English

Topic: Chemical Reaction Mechanisms

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Transition State Analysis of Enzymatic Reactions, Transition State Analysis of Enzymatic Reactions, G521541070