Work

Differential expression of laminin chains in hepatic lipocytes

Year: 1991

Type: article

Abstract: The lipocyte is an important source of laminin in the normal liver. We have investigated the expression of the 3 chains of laminin in isolated rat lipocytes. Both B1 and B2 chains, but not A, were found in medium from 5-day-old lipocyte primary cultures by immunoblotting and immunoprecipitation of 35S-labeled proteins after reducing SDS-polyacrylamide gel electrophoresis. An additional polypeptide of Mr = 380,000 was identified by immunoprecipitation. Under non-reducing conditions only one Mr = 900,000 band was revealed. High levels of B1 and B2 mRNAs were also demonstrated in 5-day-old cultured lipocytes while at the time of seeding, only B2 chain mRNAs were clearly detectable. A chain mRNA was constantly absent. These results suggest that lipocytes produce a variant form of laminin in primary culture and that the Mr = 380,000 polypeptide could be unrelated to the A chain of laminin.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)81213-r

Authors: Olivier Loreal, Françoise Levavasseur, Pierre-Yves Rescan, Yoshihiko Yamada, André Guillouzo, Bruno Clement

Institutions: Inserm, Hôpital Pontchaillou, National Institutes of Health, National Institute of Dental and Craniofacial Research

Language: English

Topic: Liver physiology and pathology

Subfield: Hepatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Institut National de la Santé et de la Recherche Médicale