Work

Equilibrium dialysis study of binding of hexammine cobalt(III) to DNA

Year: 1988

Type: article

Abstract: In recent years there has been great interest in using hexammine cobalt(II1) as an isovalent analogue of spermidine.Hexammine cobalt(II1) qualitatively mimics spermidine in many of its physical effects on nucleic acids.Quantitatively, however, there are often large differences in the effects of the two ions.Both spermidine and hexammine cobalt(II1) promote the collapse of various DNAs into compact structures.'-4Up to 5 times the amount of spermidine, compared to hexammine cobalt(III), is required to cause collapse.The B-Z transition can be driven by either ion.The transition, driven by hexammine cobalt(II1) binding, is observed in d(pCpG), ,5 poly(dG-dC), ' and poly(dG-m5dC).' Poly(dG-dC) also undergoes a B-Z-

Source: Biopolymers

DOI: 10.1002/bip.360270611

Authors: G. Eric Plum, Victor A. Bloomfield

Institution: University of Minnesota

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: G1284515461