Year: 2013
Type: article
Abstract: BACKGROUND: C-reactive protein (CRP) is a marker of inflammation and a risk predictor of cardiovascular disease. Current CRP assays are focused on the quantification of the CRP levels as pentamers. However, CRP can be present as other multimeric forms. There will be a market need to measure the CRP multimeric structure in addition to the levels in human populations. To meet this need, we investigated whether the long-term archived samples could be used instead of freshly collected samples. METHODOLOGY/PRINCIPAL FINDINGS: The specimens of serum, plasma and tissues were collected from transgenic rats expressing the human CRP. These samples were stored at 4°C, -20°C and -80°C for different periods. Non-denaturing Western blot analysis was used to observe the influence of storage conditions to multimeric structures of human CRP. Our results showed that there was no difference on multimeric structures of human CRP between samples stored at 4°C, -20°C and -80°C, between samples stored at -80°C for twenty-four hours and three months, and between plasma and serum. CONCLUSIONS/SIGNIFICANCE: This study implicated that archived samples stored at these conditions in those large longitudinal studies could be used for investigating the multimeric structures of CRP. Our report may speed up these researches and save labors and budget by enabling them to use currently available archived samples rather than freshly collected samples.
Source: PLoS ONE
Authors: Qiling Li, Ting Kang, Xiaohua Tian, Yamin Ma, Min Li, Jendai Richards, Tameka Bythwood, Yueling Wang, Xu Li, Dong Liu, Li Ma, Qing Song
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
Funders: U.S. Department of Veterans Affairs, American Heart Association, National Institutes of Health, National Heart, Lung, and Blood Institute, National Human Genome Research Institute, National Institute of General Medical Sciences, National Center for Research Resources, NIH Clinical Center, National Institute on Minority Health and Health Disparities
Awards: CENTER OF CLINICAL RESEARCH EXCELLENCE, Morehouse School of Medicine Center of Excellence Research Endowment Program, G2493439581, MSM Cardiovascular Center of Excellence, G2936269400, Determining the Phased Genome Sequences, Minority Health-GRID Network: A Genomics Resource for Health Disparity Research, G4454901344, G4648957155, Morehouse School of Medicine G12 Multidisciplinary Biomedical Research Center, G5310393447, G5604949313, Haplotype Analysis in Linkage Disequilibrium Mapping, G7285622858, ENHANCEMENT OF CARDIOVASCULAR AND RELATED RESEARCH AREAS, Establish a Transgenic Model of Human C-Reactive Protein Over-expression in Rats, G9000013296
OpenAlex ID: W1972554106