Work

Ex vivo activated and transduced human T cells generate lethal GVHD in a mouse model, and are efficiently eliminated in vivo with suicide gene therapy

Year: 2006

Type: article

Abstract: No in vivo models exist to consistently examine the impact of ex vivo manipulation of human T cells (huT) on T cell function. NOD SCIDβ2Mnull mice (β2) were conditioned with 250 cGy TBI on day −1 (n = 34), or 300 cGy on day 0 (n = 21). 107 naive or CD3/28 bead activated (Xcyte™ Dynabeads®) huT (Act) were injected retro-orbitally (ro). Engraftment of huT was evaluated weekly by FACS and euthanasia was performed if mice lost >20% body weight. β2 mice receiving 250 or 300 cGy + naive, or 300 cGy + Act developed 60%, 75%, and 100% lethal GVHD respectively.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2005.11.167