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Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group

Year: 2012
Type: article
Study design: Observational Study
Abstract: Purpose. (123)I-metaiodobenzylguanidine (MIBG) is used for the diagnostic evaluation of neuroblastoma. We evaluated the relationship between norepinephrine transporter (NET) expression and clinical MIBG uptake. Methods. Quantitative reverse transcription PCR (N = 82) and immunohistochemistry (IHC; N = 61) were performed for neuroblastoma NET mRNA and protein expression and correlated with MIBG avidity on diagnostic scans. The correlation of NET expression with clinical features was also performed. Results. Median NET mRNA expression level for the 19 MIBG avid patients was 12.9% (range 1.6-73.7%) versus 5.9% (range 0.6-110.0%) for the 8 nonavid patients (P = 0.31). Median percent NET protein expression was 50% (range 0-100%) in MIBG avid patients compared to 10% (range 0-80%) in nonavid patients (P = 0.027). MYCN amplified tumors had lower NET protein expression compared to nonamplified tumors (10% versus 50%; P = 0.0002). Conclusions. NET protein expression in neuroblastoma correlates with MIBG avidity. MYCN amplified tumors have lower NET protein expression.
DOI: 10.1155/2012/250834
Authors: Steven G. DuBois, Ethan Geier, Vandana Batra, Sook Wah Yee, John Neuhaus, Mark Segal, Daniel Martinez, Bruce Pawel, Greg Yanik, Arlene Naranjo, Wendy B. London, Susan Kreissman, David Baker, Edward Attiyeh, Michael D. Hogarty, John M. Maris, Kathleen Giacomini, Katherine K. Matthay
Institutions: University of California, San Francisco, University of California San Francisco Medical Center, Children's Hospital of Philadelphia, University of Pennsylvania, University of Michigan, Michigan Medicine, University of Florida, Children's Oncology Group, Boston Children's Hospital, Harvard University, Dana-Farber/Harvard Cancer Center, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Duke University, Duke Medical Center, Princess Margaret Hospital for Children
Language: English
Subfield: Neurology
Field: Medicine
Open Access status: hybrid
Funders: Alex's Lemonade Stand Foundation for Childhood Cancer, Dougherty Foundation, Nationwide Children's Hospital, Frank A. Campini Foundation, National Institutes of Health, University of California, San Francisco, National Cancer Institute, National Center for Research Resources
Awards: Clinical and Translational Science Institute, G1802502173, G2261000672, G2393744123, G2627126989, G2901481919, G3273505172, G3358488789, G3418010785, G4900305184, G56570703, G7299389672, G758528517, G9000058261, G9000150487, G9000166495, G9000211056, G9000215840, G9000285815, G997771125
OpenAlex ID: W1973422796
FWCI: 1.308
Cites: 30
Cited by: 61
Related to: 10