Work

Modulation of LRP6‐mediated Wnt signaling by molecular chaperone Mesd

Year: 2006

Type: article

Abstract: LRP6 is a Wnt coreceptor at the cell surface. Here, we report that a specialized molecular chaperone Mesd modulates LRP6-mediated Wnt signaling and how different LRP6 mutants exhibit differential effects on Wnt signaling. We found that overexpression of increasing amounts of the full-length LRP6 enhances Wnt signaling in a dose dependent manner only in the presence of a co-expression of the molecular chaperone Mesd, which promotes LRP6 folding and maturation to the cell surface. We also demonstrated that LRP6 mutant lacking the intracellular domain impedes LRP6 cell surface expression and Wnt signaling in a dominant-negative fashion by sequestering Mesd from promoting LRP6 folding. Our results present novel mechanisms by which Mesd and LRP6 modulate Wnt signaling.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2006.09.011

Authors: Yonghe Li, Wenyan Lu, Xi He, Guojun Bu

Institutions: Southern Research Institute, St. Louis Children's Hospital, Washington University in St. Louis, Boston Children's Hospital, Harvard University

Language: English

Topic: Wnt/β-catenin signaling in development and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Institute on Aging

Award: G4279010601