Work

NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage‐related protein family PF13554

Year: 2011

Type: article

Abstract: The solution structure of the hypothetical phage-related protein NP_888769.1 from the Gram-negative bacterium Bordetella bronchoseptica contains a well-structured core comprising a five-stranded, antiparallel β-sheet packed on one side against two α-helices and a short β-hairpin with three flexibly disordered loops extending from the central β-sheet. A homology search with the software DALI identified two Protein Data Bank deposits with Z-scores > 8, where both of these proteins have less than 8% sequence identity relative to NP_888769.1, and one has been functionally annotated as a lambda phage tail terminator protein. A sequence-homology analysis then confirmed that NP_888769.1 represents the first three-dimensional structural representative of a new protein family that was previously predicted by the Joint Center for Structural Genomics, which includes so far about 20 prophage proteins encoded in bacterial genomes.

Source: Protein Science

DOI: 10.1002/pro.641

Authors: Atia‐tul‐ Wahab, Pedro Serrano, Michael Geralt, Kurt Wüthrich

Institutions: Scripps Research Institute, University of Karachi, Joint Center for Structural Genomics, International Center for Chemical and Biological Sciences

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: University of Karachi, National Institutes of Health, National Institute of General Medical Sciences

Awards: Joint Center for Structural Genomics, Joint Center for Structural Genomics (JCSG-2)