Work

Vibrationally resonant sum-frequency generation microscopy with a solid immersion lens

Year: 2014

Type: article

Abstract: We use a hemispheric sapphire lens in combination with an off-axis parabolic mirror to demonstrate high-resolution vibrationally resonant sum-frequency generation (VR-SFG) microscopy in the mid-infrared range. With the sapphire lens as an immersed solid medium, the numerical aperture (NA) of the parabolic mirror objective is enhanced by a factor of 1.72, from 0.42 to 0.72, close to the theoretical value of 1.76 ( = nsapphire). The measured lateral resolution is as high as 0.64 μm. We show the practical utility of the sapphire immersion lens by imaging collagen-rich tissues with and without the solid immersion lens.

Source: Biomedical Optics Express

DOI: 10.1364/boe.5.002125

Authors: Eun Seong Lee, Sang-Won Lee, Julie Hsu, Eric O. Potma

Institutions: Korea Research Institute of Standards and Science, University of California, Irvine

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,913