Work

Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA

Year: 1974

Type: article

Abstract: A bacteriophage lambda strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the lambda DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this lambda strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.71.11.4579

Authors: Marjorie Thomas, John R. Cameron, Ronald W. Davis

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1973856483

FWCI: 18.12

Cites: 10

Cited by: 238

Related to: 10