Work

Microbeam-coupled capillary electrophoresis

Year: 2015

Type: article

Abstract: Within the first few microseconds following a charged particle traversal of a cell, numerous oxygen and nitrogen radicals are formed along the track. Presented here is a method, using capillary electrophoresis, for simultaneous measurement, within an individual cell, of specific reactive oxygen species, such as the superoxide radical ([Formula: see text]) as well as the native and oxidised forms of glutathione, an ubiquitous anti-oxidant that assists the cell in coping with these species. Preliminary data are presented as well as plans for integrating this system into the charged particle microbeam at Columbia University.

Source: Radiation Protection Dosimetry

DOI: 10.1093/rpd/ncv148

Authors: G. Garty, M. U. Ehsan, M. Buonanno, Z. Yang, J. V. Sweedler, D. J. Brenner

Institutions: Columbia University, University of Illinois Urbana-Champaign

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funders: National Science Foundation, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering, Division of Chemistry

Awards: Radiological Research Accelerator Facility (RARAF), Bioanalytical Characterization of D-Amino Acids in the Brain