Bmp2 gene in osteoblasts of periosteum and trabecular bone links bone formation to vascularization and mesenchymal stem cells
Year: 2013
Type: article
Abstract: We generated a new Bmp2 conditional-knockout allele without a neo cassette that removes the Bmp2 gene from osteoblasts (Bmp2-cKO(ob)) using the 3.6Col1a1-Cre transgenic model. Bones of Bmp2-cKO(ob) mice are thinner, with increased brittleness. Osteoblast activity is reduced as reflected in a reduced bone formation rate and failure to differentiate to a mature mineralizing stage. Bmp2 in osteoblasts also indirectly controls angiogenesis in the periosteum and bone marrow. VegfA production is reduced in Bmp2-cKO(ob) osteoblasts. Deletion of Bmp2 in osteoblasts also leads to defective mesenchymal stem cells (MSCs), which correlates with the reduced microvascular bed in the periosteum and trabecular bones. Expression of several MSC marker genes (α-SMA, CD146 and Angiopoietin-1) in vivo, in vitro CFU assays and deletion of Bmp2 in vitro in α-SMA(+) MSCs support our conclusions. Critical roles of Bmp2 in osteoblasts and MSCs are a vital link between bone formation, vascularization and mesenchymal stem cells.
Source: Journal of Cell Science
DOI: 10.1242/jcs.118596
Authors: W. Yang, D. Guo, M.A. Harris, Y. Cui, J. Gluhak-Heinrich, J. Wu, X.-D. Chen, C Skinner, J. Nyman, J.R. Edwards, G.R. Mundy, A. Lichtler, B. Kream, D. Rowe, I. Kalajzic, V. David, D. Quarles, D. Villareal, Greg Scott, Manas Ray, S. Liu, J.F. Martin, Y. Mishina, S.E. Harris
Institutions: The University of Texas at San Antonio Health Science Center, UConn Health, Regeneron (United States), University of Missouri–Kansas City, Vanderbilt University, Vanderbilt University Medical Center, University of Tennessee Health Science Center, National Institute of Environmental Health Sciences, Baylor College of Medicine, University of Michigan
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1974550900