HIV-1 infection of hematopoietic progenitor cells in vivo in humanized mice
Year: 2013
Type: article
Abstract: HIV infection has been associated with defective hematopoiesis since the earliest days of the HIV/AIDS epidemic. Generation of all hematopoietic lineages suffers in the face of infection. The mechanisms by which HIV impairs normal blood cell development remain unclear, and direct infection of intermediate hematopoietic progenitors has not been established as a source of HIV-associated hematopoietic pathology. Here, we demonstrate infection of multiple subsets of highly purified intermediate hematopoietic progenitors by wild-type HIV both in vitro and in vivo. Although direct infection is clearly cytotoxic, we find that some infected progenitors can survive and harbor proviral DNA. We report intermediate hematopoietic progenitors to be a novel target of infection and their permissivity to infection increases with development. Further, the nonobese diabetic severe combined immunodeficiency common γ chain knockout-bone marrow-liver-thymus humanized mouse provides a unique model for studying the impact of HIV infection on bone marrow-based human hematopoiesis.
Source: Blood
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Awards: G1345503394, Interdisciplinary Training in Virology and Gene Therapy, Effects of cocaine on HIV infection of quiescent T cells, Targeting HIV Reservoirs in vitro and in vivo, HIV Infection of Hematopoietic Stem/Progenitor Cells (HSPC) in Bone Marrow, UCLA Center for AIDS Research (CFAR), Martin Delaney Collaboratory to Eradicate HIV-1 Infection
OpenAlex ID: W1974644145