Work

The gene encoding ARS-binding factor I is essential for the viability of yeast.

Year: 1989

Type: preprint

Abstract: The gene encoding a yeast ARS-binding protein, ABF I, has been cloned by screening a genomic lambda gt11 library using monoclonal and polyclonal antibodies against ABF I. ABF I is of interest because it not only binds to ARSs but also to the 5'-flanking region of genes encoding proteins involved in transcription, translation, respiration, and cell-cycle control. The cloned gene has been used to prepare null mutants, which further demonstrate the importance of the ABF I protein by showing that it is essential for vegetative growth. ABF1 maps to chromosome V. The DNA sequence of the ABF1 gene reveals several motifs characteristic of DNA-binding proteins but shows no overall similarity to any protein of known function.

Source: Genes & Development

DOI: 10.1101/gad.3.12a.1926

Authors: P R Rhode, K S Sweder, K F Oegema, J L Campbell

Institution: California Institute of Technology

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze