Work

Inhibition of RNA polymerase II transcription by oligonucleotide-RecA protein filaments targeted to promoter sequences.

Year: 1993

Type: article

Abstract: In the presence of RecA protein, which plays a major role in genetic recombination in Escherichia coli, an oligodeoxyribonucleotide can find its homologous counterpart in double-stranded DNA and form triple-stranded structures. A triple-stranded structure formed by an oligonucleotide with a sequence overlapping essential regulatory elements of a viral promoter, such as TATA or GC boxes, inhibited in vitro transcription driven by RNA polymerase II. An oligonucleotide with eight nucleotides homologous to its target suppressed RNA polymerase II activity in HeLa cell extracts. This procedure offers a potential alternative to the usual mutational analysis of transcriptional promoters.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.90.15.7186

Authors: E I Golub, C M Radding, D C Ward

Institution: Yale University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences

Awards: AFFINITY PURIFICATION OF LARGE FRAGMENTS OF HUMAN DNA, G2793380339, RNA-DNA HYBRIDIZATION BY E. COLI RECA PROTEIN, G4418560981