Work

T cell receptor V beta gene bias in rheumatoid arthritis.

Year: 1993

Type: article

Abstract: Polymerase chain reaction (PCR) technology was employed to examine peripheral blood and synovial T cells in patients with rheumatoid arthritis (RA) for biased utilization of T cell receptor (TCR) variable region (V) genes.Oligonucleotide primers specific for individual TCR V,6 gene families were used to am- plify TCR gene products in a semiquantitative assay of their relative utilization in unselected T cell populations.Mean VB expression in 24 RA peripheral blood samples was very similar to that in a panel of 15 normal subjects, except for a slight decrease in V,813.2 expression.VjO utilization in 8 RA synovial tissue samples and 13 synovial fluid samples was compared to simultaneously obtained blood samples.Although heterogeneous patterns of skewed V,@ utilization were observed, several significant trends emerged.By a number of approaches to data analysis, a statistically significant increase in expression of V/i6 and V#I5 in synovial T cells was documented.In addition, increased synovial expression of V/i14 was found, but only in the synovial fluid samples.Reduced expression of V#1, Vj64, V/i5.1, V/i10, V,816, and V/i19 was also observed in synovial T cells.These results indicate that biased V,8 gene utilization in different peripheral compartments of RA patients can be ob- served in unselected T cell populations, and are consistent with the conclusion that populations of T cells expressing these V/i gene products may be involved in the pathogenesis of the dis- ease.(J.Clin.Invest.1993.92:2688-2701.)Key words: polymerase chain reaction * rheumatoid arthritis -synovial T cells-T cell receptor * T cell repertoire Vfl6.1-3, V#31 5, and Vf l9 was at least 50% greater than that of peripheral blood in this patient (RA-23).On the other hand, Vf32, V,35. 1, VB9, V,3I0, Vf I 1, V 12, and V#316 expression was at least 50% greater in peripheral blood.The number ofcases in which expression ofa particular Vfl 2694 Jenkins et al.Vo1 V,32 V#3 V#4 V35. 1 Vf35.2-3Vi36.1-3Vfl7 VB8 V#9 VolO Vo1 1 V#12 Vf3 13.1 Vf13.2 Vf314 V#15 V#16 V#317 Vf318 Vfli19 V#20 24 3 SFand PB Vf7 in 2 Va: no 14 9

Source: Journal of Clinical Investigation

DOI: 10.1172/jci116886

Authors: R N Jenkins, A Nikaein, A Zimmermann, K Meek, P E Lipsky

Institutions: The University of Texas Southwestern Medical Center, Baylor University Medical Center

Language: English

Topic: Rheumatoid Arthritis Research and Therapies

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Arthritis Foundation, National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases