Year: 2011
Type: article
Abstract: The blood-brain barrier (BBB) is a multicellular vascular structure separating blood from the brain parenchyma that is composed of endothelial cells with tight intercellular junctions, surrounded by a basal lamina, astrocytes, and pericytes. Previous studies have generated detailed databases of the microvessel transcriptome; however, less information is available on the BBB at the protein level. In this study, we specifically focused on characterization of the membrane fraction of cells within the BBB to generate a more complete understanding of membrane transporters, tight junction proteins, and associated extracellular matrix proteins that are functional hallmarks of the BBB. We used Multidimensional Protein Identification Technology to identify a total of 1,143 proteins in mouse brain microvessels, of which 53% were determined to be membrane associated. Analyses of specific classes of BBB-associated proteins in the context of recent transcriptome reports provide a unique database to assess the relative contribution of genes at the level of both RNA and protein in the maintenance of normal BBB integrity.
Authors: Hyun Bae Chun, Michael Scott, Sherry Niessen, Heather Hoover, Andrew Baird, John Yates, Bruce E Torbett, Brian P Eliceiri
Language: English
Subfield: Neurology
Field: Neuroscience
Domain: Life Sciences
Open Access status: bronze
Funders: National Institutes of Health, Center for AIDS Research, University of Washington, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke
Awards: Host-mediated targets in glioma invasion, Role of Src family kinases in endothelial cell biology, G488505308, G5202045387, Vascular mechanisms regulating breast cancer brain metastasis, G8259150102, A Combinatorial Approach to Wound Healing for Protein, Gene and Cell Therapeutics, San Diego Center for AIDS Research (SD CFAR)
OpenAlex ID: W1975936189