Work

A semi-quantitative high-throughput screening method for microbial l-tyrosine production in microtiter plates

Year: 2007

Type: article

Abstract: In this study, we developed a fluorescence-based assay for quantifying the aromatic amino acid L-tyrosine in small sample volumes employing 96-well microtiter plates. The method was based on the specific derivatisation of L-tyrosine with 1-nitroso-2-naphthol and the formation of a stable complex for the fluorescent determination of L-tyrosine concentrations. The original procedure for L-tyrosine measurements in blood or tissue samples (Waalkes and Udenfriend in J Lab Clin Med 50:733-736, 1957) was modified to a simple assay suitable for high-throughput screening of L-tyrosine producing microorganisms such as engineered Escherichia coli.

Source: Journal of Industrial Microbiology & Biotechnology

DOI: 10.1007/s10295-007-0257-x

Authors: Tina Lütke-Eversloh, Gregory Stephanopoulos

Institution: Massachusetts Institute of Technology

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Deutsche Forschungsgemeinschaft

OpenAlex ID: W1976016203