Year: 2001
Type: review
Abstract: The use of EM and image analysis techniques, particularly the single particle methodology, has opened the door to the structural characterization of large protein complexes in the megadalton range. This review covers the basic principles of single particle EM and recent examples of its application to the study of large molecular machines in the eukaryotic cell. These examples will provide the reader a perspective on how this emerging technique can be applied to the study of large protein complexes to determine their overall shape, or the location of subunits and domains within them, and to follow conformational changes accompanying intermediates in their functional cycle.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.152.1.f1
Authors: Eva Nogales, Nikolaus Grigorieff
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1976389850