Year: 2003
Type: article
Abstract: The effects of different cotyledon ages (6, 15, 21 hours and 3 day), cotyledon positions (distal and proximal parts of the cotyledon) and growth regulators (BA and zeatin) on regeneration were tested for swelling and shoot formation. The majority of explants in all treatments (70–94%) showed evidence of swelling at 10 days. Shoot formation was observed at the 20th day. Younger seeds gave a significantly higher regeneration than older seeds on shoot formation. Shoot formation ratio was similar in 6, 15 and 21 h. age explants, but the ratio for 3 days old seeds was the worst one. Proximal explants of cotyledons from younger seeds (6, 15, 21 h) produced significantly more shoots (2–2.7 shoots/explant) than distal explants (0.75–1.5 shoots/explant). Proximal parts of the older cotyledons (3 days) produced more shoots (0.75 shoots/explant) than distal parts (0.25 shoots/explant). There were no significant difference between BA and zeatin on shoot formation of regeneration.
Author: N. Yesim Yalcin-Mendi
Institution: Cukurova University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
Funder: University of Otago
OpenAlex ID: W1976519889