Imaging workflow and calibration for CT-guided time-domain fluorescence tomography
Year: 2011
Type: article
Abstract: In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
Source: Biomedical Optics Express
DOI: 10.1364/boe.2.003021
Authors: Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, Qun Zhu, Hamid Dehghani, Frederic Leblond, Brian W. Pogue
Institutions: Dartmouth College, University of Birmingham
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,913
Funder: National Cancer Institute
OpenAlex ID: W1976616137