Work

Label‐Free Detection of Single‐Base Mismatches in DNA by Surface‐Enhanced Raman Spectroscopy

Year: 2011

Type: article

Abstract: Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single-base mismatches in short (25-mer and 23-mer) strands. Subtracting the spectra of different DNA sequences results in difference spectra that contain features corresponding to the exchanged nucleotides.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201102776

Authors: Evanthia Papadopoulou, Steven E. J. Bell

Institution: Queen's University Belfast

Language: English

Topic: Gold and Silver Nanoparticles Synthesis and Applications

Subfield: Electronic, Optical and Magnetic Materials

Field: Materials Science

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W1976707311

FWCI: 5.471

Cites: 13

Cited by: 199

Related to: 10