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Strategies for the Rapid Construction of Conditionally-Replicating HSV-1 Vectors Expressing Foreign Genes as Anticancer Therapeutic Agents

Year: 2010

Type: article

Abstract: Conditionally replication-competent Herpes Simplex Virus Type 1 (HSV-1) vectors expressing foreign genes have been developed as experimental therapeutic agents. Traditional methods of virus construction, including growth selection based on thymidine kinase gene expression, and color selection based on a reporter gene expression are often time-consuming and relatively inefficient. This review summarizes the various strategies developed in recent years for the rapid and efficient construction of novel conditionally replication-competent mutant HSV expressing multiple foreign genes. Additionally, two new modifications of existing strategies, which have not been previously reported, are discussed.

Source: Molecular Pharmaceutics

DOI: 10.1021/mp100230y

Authors: Jacqueline N. Parker, Xiaojia Zheng, William Luckett, James M. Markert, Kevin A. Cassady

Institutions: University of Alabama at Birmingham, UAB Medicine

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Neurological Disorders and Stroke