Work

Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage

Year: 1999

Type: article

Abstract: A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or gamma irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.96.5.1921

Authors: Elke Raderschall, Efim I. Golub, Thomas Haaf

Institutions: Yale University, Max Planck Institute for Molecular Genetics

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Deutsche Forschungsgemeinschaft, National Institutes of Health, National Institute of General Medical Sciences