Year: 1985
Type: article
Abstract: In the continuing search for fungal cultures having cell proliferation inhibitors,1>2) it was found that a culture extract of Aspergillus candidus showed pronounced inhibitory activity against sea urchin embryo cells.In the previous paper in this series, we reported new pterphenyls, candidusins A and B, as effective inhibitors of sea urchin embryonic development.Closer examination of the metabolites of Asp.candidus showed that the fungus produced three active compounds besides candidusins A andB.This paper deals with the isolation and characterization of the three bioactive compounds, together with the inhibitory effects on the embryosystem.Aspergillus candidus was inoculated on potato-dextroseagar plates (pH 5.0; dextrose content, 2%) and incubated for three weeks at 28°C.The cultures obtained from 100 plates (9cm O.D.) were soaked in acetone for a week at roomtemperature.The acetone solution was concentrated to give a brownish oil (1.5 g), which was subjected to silica gel column chromatography.The column was eluted stepwise with toluene-acetone in an increasing order of
Authors: Akio KOBAYASHI, Akira TAKEMOTO, Koichi KOSHIMIZU, Kazuyoshi KAWAZU
Institutions: Okayama University, Kyoto University
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W1978437029