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Sensitive Assay for Proteases in Sterile Milk

Year: 1979

Type: article

Abstract: A sensitive assay for protease activity based on the reaction of primary amino groups of trichloroacetic acid-soluble peptides and amino acids with fluorescamine was applied to sterile milk.The assay was linear in the range of 2 to 50 nmol of amino groups p~r aliquot.The assay is suitable for determining proteolytic activity in sterile milk products and for determining protease activity in general.

Source: Journal of Dairy Science

DOI: 10.3168/jds.s0022-0302(79)83499-2

Authors: G.W. Chism, A.E. Huang, J.A. Marshall

Institution: The Ohio State University

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: National Science Foundation