Automated crystal screening for high-throughput X-ray crystallography at Stanford Synchrotron Radiation Laboratory
Year: 2004
Type: article
Abstract: Macromolecular crystallography has proven to be the most effective method of determining the structures of biological macromolecules.Each year thousands of data collections that form the basis for such structure determinations are carried out at the ESRF.To cope with this high demand, it is essential to maximise beamline efficiency and to simplify beamline control procedures.The ESRF, in collaboration with the EMBL Grenoble Outstation, are therefore automating much of the crystallographic experiment which may be divided into two parts : beamline and crystal alignment, and data collection and processing.Recent progress in automatic crystal mounting and centering [1], crystal characterisation [2], absorption edge scanning and automated alignment procedures for the beamline optical elements will be described, as will the development of databases designed to aid the automation process.
Authors: A. M. Deacon, A. E. Cohen, P. J. Ellis, S. McPhillips, T. M. McPhillips, M. D. Miller, R. P. Phizackerley, J. Song, S. M. Soltis, I. Tsyba, H. van den Bedem, G. Wolf, Z. Zhang
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W1978715728