Work

The Use of pHluorins for Optical Measurements of Presynaptic Activity

Year: 2000

Type: article

Abstract: Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.

Source: Biophysical Journal

DOI: 10.1016/s0006-3495(00)76468-x

Authors: Sethuraman Sankaranarayanan, Dino De Angelis, James E. Rothman, Timothy A. Ryan

Institutions: Cornell University, Weill Cornell Medicine, Memorial Sloan Kettering Cancer Center

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,000

Funder: National Institute of Neurological Disorders and Stroke