Responses to the soluble flagellar protein FliC are Th2, while those to FliC on Salmonella are Th1
Year: 2004
Type: article
Abstract: Features of the Th1 or Th2 phenotype start to develop during CD4 T cell priming. This study of the response to the bacterial flagellar protein FliC shows that either Th1 or Th2 responses can be induced in mice depending upon how FliC is presented. This is shown by assessing the cytokine mRNA and class of FliC-specific plasma cells induced in situ. Soluble recombinant (r)FliC and polymerized FliC are strongly Th2 polarizing, inducing IL-4, NIP45 and c-Maf mRNA as well as epsilon and gamma1 switch transcripts and switching to IgG1. CD28-requirement for this switching shows its T cell dependence. rFliC was unable to induce markers of Th1 activity including IL-12, T-bet and IFN-gamma. Conversely, when FliC is presented in its native context surface-bound on live, flagellated Salmonella, switching is predominantly to IgG2a (IgG2c in C57BL/6 mice), reflecting Th1 activity. The development of divergent FliC-specific polarization to either Th1 or Th2 indicates that the context in which this antigen is encountered rather than its intrinsic immunostimulatory properties determines the direction of Th polarization.
Source: European Journal of Immunology
Institution: University of Birmingham
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1978897589